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Thermo Scientific™ Phusion™ HF and GC Buffer Packs
5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.
Marke: Thermo Scientific™ F518L
32.66 CHF gültig bis 2024-12-20
Benutzen Sie den Promotions-Code "21651" um Ihren Promotions-Preis zu erhalten.
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Beschreibung
The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.
HF-Puffer für Phusion DNA-Polymerasen, geeignet für alle Standard-TemplatesEnthält:
5X Phusion HF-Puffer, 50 mM MgCl2-Lösung, 100 % DMSO.
Spezifikation
| Das Phusion HF-Puffer-Pack (F-518L) enthält: • 5X Phusion HF-Puffer (1X Puffer liefert 1,5 mM MgCl2) • 50 mM MgCl2 • DMSO Bei -20 °C lagern. |
|
| Phusion High-Fidelity DNA-Polymerase | |
| Nass- oder Trockeneis | |
| DNA | |
| Colorless |
| Phusion™ HF-Pufferpaket | |
| HF Buffer Pack | |
| Standard-PCR, PCR mit hoher Wiedergabetreue | |
| 4 × 1,5 ml |
For Research Use Only. Not for use in diagnostic procedures.