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Thermo Scientific™ Phusion™ HF and GC Buffer Packs
5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.
Marke: Thermo Scientific™ F521L
32.66 CHF gültig bis 2024-12-20
Benutzen Sie den Promotions-Code "21651" um Ihren Promotions-Preis zu erhalten.
Beschreibung
The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.
Tensidfreier HF-Puffer für Phusion DNA-Polymerasen Entwickelt für DHPLC- und Microarray-AnwendungenEnthält:
Detergensfreier 5X Phusion HF-Puffer, 50 mM MgCl2-Lösung, 100 % DMSO.
Spezifikation
Enthält: • 5X Phusion GC-Puffer, ohne Detergenzien (1X Puffer liefert 1,5 mM MgCl2) • 50 mM MgCl2 • DMSO Bei -20 °C lagern. |
|
Phusion High-Fidelity DNA-Polymerase | |
Nass- oder Trockeneis | |
DNA | |
Colorless |
Paket detergensfreie Phusion™ GC-Puffer | |
Detergent-free GC Buffer Pack | |
Standard-PCR, PCR mit hoher Wiedergabetreue | |
4 × 1,5 ml |
For Research Use Only. Not for use in diagnostic procedures.