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    1. Home
    2. Molecular Biology Methods

    Molecular Biology Methods

    Molecular biology techniques are used to investigate the structure and function of the cells and tissues of living organisms. These techniques may include DNA and RNA analysis, gene expression, biochemical analysis, and protein production. Find DNA and RNA testing products, including reagents for locating specific DNA sequences, as well as products for transfection, chemical and electroporation transformations, nucleic acid labeling and detection, and more.

    Overview
    1. Isolation and Purification
    2. Labeling and Detection
    3. Amplification
    4. Cloning and Modification
    5. Transfection
    6. Analysis
    Related Resources
    Other Applications

    Molecular Biology Methods

    1. Isolation and Purification


    Well-prepared nucleic acids are critical to your experiments and research, so how you isolate and purify your samples is important. Multiple methods exist for disrupting the cells, removing debris, capturing your sample, removing contaminants, and eluting the sample for use in downstream applications. Find products for isolation, extraction, purification, and more to complete this step of your process.

    Top Categories

    DNA Extraction and Purification

    DNA Extraction and Purification

    Molecular Biology Reagents and Kits

    Molecular Biology Reagents and Kits

    Nucleic Acid Purification Systems

    Nucleic Acid Purification Systems

    Featured Products

    Placeholder

    Catalog Number 16416159

    Cytiva Sera-Xtracta Cell-Free DNA Kit
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    Catalog Number 12703057

    MP Biomedicalsâ„¢ FastRNAâ„¢ Pro Soil-Direct Kit
    Viewing 0-2 of 2

    2. Labeling and Detection


    Tags or labels, called probes, are added to nucleic acids to enable detection or purification. Labels may include radioactive phosphates, biotin, fluorophores, and enzymes. Fluorescent in situ hybridization (FISH) is used to identify specific DNA sequences and tests can also be performed for pathogen detection. Find labeling and detection kits, spectrophotometers, fluorometers, and more below for your nucleic acid work.

    Top Categories

    Nucleic Acid Labeling and Detection

    Nucleic Acid Labeling and Detection

    UV-Vis Spectrophotometers

    UV-Vis Spectrophotometers

    Fluorometers and Fluorescence Polarization Systems

    Fluorometers and Fluorescence Polarization Systems

    Featured Products

    Placeholder

    Catalog Number 7235448

    Invitrogenâ„¢ Qubitâ„¢ Flex Starter Packages
    Placeholder

    Catalog Number 6909043

    Thermo Scientificâ„¢ GENESYSâ„¢ 30 Visible Spectrophotometer
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    Catalog Number 3676034

    Cytiva Amershamâ„¢ ECLâ„¢ Direct Nucleic Acid Labeling/Detection System
    Viewing 1-3 of 3

    3. Amplification


    The goal of PCR is the amplification of existing DNA. Ultimately, having multiple copies makes it possible to perform your assays. Choose from a variety of thermal cyclers, polymerases, master mixes, and other PCR systems to support your PCR amplification needs.

    Top Categories

    Thermal Cyclers

    Thermal Cyclers

    DNA Polymerases

    DNA Polymerases

    PCR Supplies

    PCR Supplies

    Featured Products

    Placeholder

    Catalog Number 15304502

    Applied Biosystemsâ„¢ ProFlexâ„¢ PCR System, 2 x 384-well
    Placeholder

    Catalog Number 7184433

    Thermo Scientificâ„¢ dNTP Mix (25 mM each)
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    4. Cloning and Modification


    Cloning and genetic modification can be used to research and identify genetic defects. Cell and gene therapy are expanding fields of interest. Choose from a variety of competent cells, PCR cloning kits, enzymes and inhibitors, and more.

    Top Categories

    Competent Cells

    Competent Cells

    PCR Cloning Kits

    PCR Cloning Kits

    Enzymes and Inhibitors

    Enzymes and Inhibitors

    Featured Products

    Placeholder

    Catalog Number 4521711

    Thermo Scientificâ„¢ FastDigest EcoRI
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    Catalog Number 16575231

    Invitrogenâ„¢ GeneArtâ„¢ Gibson Assembly HiFi Cloning Kit, chemically competent cells
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    Catalog Number 15930583

    Invitrogenâ„¢ MultiShotâ„¢ FlexPlate TOP10 Competent Cells
    Viewing 1-3 of 3

    5. Transfection


    Mimicking natural viral processes, transfection artificially introduces nucleic acids to cells, producing various chemical, biological, or physical changes. Although the term transfection was originally used to describe the uptake of viral nucleic acid from a prokaryote‑infecting virus or bacteriophage, the term currently refers to any artificial introduction of foreign nucleic acid into a cell. Search for transfection reagents, electroporation supplies, and more to help effectively conduct your transfection procedures.

    Top Categories

    Transfection Reagents

    Transfection Reagents

    Electroporators

    Electroporators

    Electroporation Cuvets

    Electroporation Cuvets

    Featured Products

    Placeholder

    Catalog Number 15898441

    BTXâ„¢ ECMâ„¢ 630 Exponential Decay Wave Electroporation System Complete, with Safety Stand
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    Catalog Number 10431915

    Invitrogenâ„¢ Neonâ„¢ Transfection System
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    Catalog Number 6960076

    Invitrogenâ„¢ Invivofectamineâ„¢ 3.0 Reagent
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    6. Analysis


    The yield from your PCR and molecular biology procedures must be further analyzed and evaluated to confirm the outcome of your assays, especially if the material is intended for other downstream applications. Shop electrophoresis equipment, gel stains, imaging and documentation systems, and more to help ensure the success of your molecular biology assays.

    Top Categories

    Gel Electrophoresis Equipment and Supplies

    Gel Electrophoresis Equipment and Supplies

    Electrophoresis Gel Stains

    Electrophoresis Gel Stains

    Gel Imaging and Documentation Systems

    Gel Imaging and Documentation Systems

    Featured Products

    Placeholder

    Catalog Number 16514341

    Syngeneâ„¢ Airclean 600 PCR Workstation
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    Catalog Number 15879922

    Syngeneâ„¢ G:BOX - F3 with Feedback Lens
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    Catalog Number 10328162

    Invitrogenâ„¢ SYBRâ„¢ Safe DNA Gel Stain
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    Related Resources


    Laboratory Essentials

    Laboratory Essentials, for Your Life Science Workflow

    Download the PDF
    Laboratory essentials, for your life science workflow

    Vaccines: BioPharma Application

    Learn More
    Vaccines: BioPharma Application

    The Promise of Oligos in Medicine

    Learn More
    The Promise of Oligos in Medicine

    Isolation and Sequencing

    Learn More
    Isolation and Sequencing
    Viewing 1-3 of 4

    Other Applications


    Cell Analysis Methods

    Discover More
    Cell Analysis Methods

    PCR and qPCR

    Discover More
    PCR and qPCR

    Cell Culture

    Discover More
    Cell Culture

    Top Brands


    Applied Biosystems
    Corning
    Cytiva
    Eppendorf
    Fisher Bioreagents
    Fisherbrand
    Invitrogen
    MP
    Thermo Scientific
    Viewing 1-6 of 9
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