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Gibco™ RPMI 1640 Medium, niedrige HEPES, niedriges Bikarbonat, ohne Glutamin
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
Marke: Gibco™ 42402016
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Beschreibung
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI 1640 is modified as follows:
- With: Phenol Red, HEPES
- Without: L-glutamine
The complete formulation is available.
Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.
Product Use
RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Spezifikation
| Gemäß Norm ISO 13485, FDA | |
| CO2 | |
| Ohne tierische Bestandteile | |
| Säugetierzellkultur | |
| RPMI 1640 | |
| Steril gefiltert | |
| Kein Glutamin, Ohne Natriumpyruvat | |
| Nachhaltige Verpackung | |
| 270 bis 300 mOsm/kg | |
| Gibco™ | |
| Raumtemperatur |
| Lagerbedingungen: 2 bis 8 °C. Vor Licht schützen. Versandbedingungen: Umgebungstemperatur. Haltbarkeit: 12 Monate ab Herstellungsdatum. |
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| Roswell Park Memorial Institute (RPMI) 1640 Medium, 500 ml, flüssig, 1x Konzentration, pH 7,0 bis 7,4, mit Phenolrot, HEPES, Haltbarkeit 12 Monate, Osmolalität 270 bis 300 mOsm/kg, für Säugetierzellkulturen | |
| 1 X | |
| Flüssig | |
| Standard-Serumzugabe | |
| HEPES, Phenolrot, Natriumhydrogencarbonat | |
| Säugetierzellkultur | |
| cGMP-compliant under the ISO 13485 standard | |
| 7 bis 7,4 | |
| 500 mL |
Nur für Forschungszwecke. Nicht für diagnostische oder therapeutische Zwecke an Tieren und Menschen geeignet.