Allgemeine zellbasierte Assays

Allgemeine zellbasierte Assays
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Thermo Scientific™ Kinase Tracer 236, 50 μM
Im LanthaScreen™ Eu-Kinase-Bindungsassay wird die Bindung eines Alexa Fluor™ Konjugats oder einer Markierungssubstanz wie der Kinase Markierungssubstanz 236 an eine Kinase durch Zugabe eines mit Eu markierten Anti-Tag-Antikörpers nachgewiesen.
Thermo Scientific™ LanthaScreen™ STAT1 U2OS Cell Line
The JAK/STAT1 signaling pathway is known to be activated by type I/II interferons such as interferon-gamma and interferon-alpha.
Thermo Scientific™ Tango™ AGTRL1-bla U2OS Cells
The Tango™ AGTRL1-bla U2OS cells contain the human Angtiotension-Like Receptor 1 (AGTRL1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.
Thermo Scientific™ CellSensor™ CRE-bla Jurkat-Zelllinie
The CRE-bla Jurkat cell line contains a beta-lactamase reporter gene under control of a cAMP response element (CRE) stably integrated into Jurkat cells.
Thermo Scientific™ LanthaScreen™ STAT5 TF-1-Zelllinie
LanthaScreen™ GFP Cellular Assays allow for the analysis of post-translational modifications for a number of target proteins in an live-cell context.
Thermo Scientific™ CellSensor™ TrkA-NFAT-bla CHO-K1-Zelllinie
Neurotrophins (NGF, BDNF, NT-3, and NT-4) and their transmembrane receptors (TrkA, TrkB, TrkC, and P75NTR) play important roles in the regulation of neuronal and non-neuronal cell proliferation, differentiation, survival, and death.
Thermo Scientific™ GeneBLAzer™ GR-DA-Assay-Kit
GeneBLAzer™GR DA (Division Arrested) cells and GR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Gluocorticoid receptor (GR) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.
Thermo Scientific™ GeneBLAzer™ Gqi5-NFAT-bla FreeStyle™ 293F Cells
GeneBLAzer™ Gqi5-NFAT-bla FreeStyle™ 293F cells contain the chimeric G-protein Gqi5 stably integrated into the CellSensor™ NFAT-blaFreeStyle™ 293F cell line.
Thermo Scientific™ GeneBLAzer™ CCKBR HEK293T-DA-Assay Kit
GeneBLAzer™ CCKBR HEK 293T DA (Division Arrested) cells and CCKBR-NFAT-bla HEK 293T cells contain the human Cholecystokinin (CCKBR) receptor stably integrated into the CellSensor™ NFAT-bla HEK 293T cell line.
Thermo Scientific™ GeneBLAzer™ FPRL-1 -Gα15 CHO-K1 DA Assay-Kit
GeneBLAzer™ FPRL-1-Gα15 CHO-K1 DA (Division Arrested) cells and FPRL-1-Gα15-NFAT-bla CHO-K1 cells contain the human Formyl Peptide-like 1 receptor (FPRL-1) stably integrated into the GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cell line.
Thermo Scientific™ GeneBLAzer™ FXR-UAS-bla HEK 293T-Zellen
GeneBLAzer™ EDG7-NFAT-bla HEK293T cells contain the human Endothelial Differentiation Gene (EDG7) stably integrated into the CellSensor™ NFAT-bla HEK293T cell line.
Thermo Scientific™ Tango™ EDG3-bla U2OS Cells
Tango™ EDG3-bla U2OS cells contain the human Endothelial Differentiation Gene 3 (EDG3) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.
Thermo Scientific™ CellSensor™ CRE HEK 293T DA Assay Kit
The CellSensor™ CRE HEK 293T DA (Division Arrested) cells contains a beta-lactamase (bla) reporter gene under control of a cAMP response element (CRE) stably integrated into HEK293T cells.
Thermo Scientific™ LanthaScreen™ ERK2 A375 Cell Line
The Map Kinase (MapK) signal transduction cascade is activated by growth factors such as EGF, PDGF, and HGF.
Thermo Scientific™ CellSensor™ NFAT CHO-K1 DA Assay-Kit
The CellSensor™ NFAT CHO-K1 DA (Division Arrested) cells contain a beta-lactamase (bla) reporter gene under control of a Nuclear Factor of Activated T Cells (NFAT) response element stably integrated into CHO-K1 cells.