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R&D Systems™ Recombinant Mouse BID (Caspase-8-cleaved) Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
Marke: R&D Systems™ 883-M8-050
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Beschreibung
Measured by its ability to induce cytochrome c release from isolated mouse liver mitochondria. The typical EC50 for cytochrome c releasing activity is <100 nM. Cytochrome c is quantified using the Rat/Mouse Cytochrome c Quantikine™ ELISA Kit (Catalog # MCTC0 ). The EC50 for the desired application should be determined. Uncleaved Recombinant Mouse BID is available (Catalog # 860-MB ).
Spezifikation
P70444 | |
Supplied as a 0.2 μm filtered solution in HEPES-KOH and KCl. | |
M.W. (Observed): 7 kDa and 15 kDa, under reducing and non-reducing conditions; M.W. (theoretical): 7 kDa (N-terminal fragment) & 15 kDa (C-terminal fragment) | |
50 μg | |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening. | |
Unconjugated | |
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
Bioactivity | |
637 | |
BID Caspase-8-cleaved | |
E. coli-derived mouse BID protein Met1-Asp195 | |
Bid | |
Recombinant | |
BID |
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R&D Systems™ Recombinant Mouse BID (Caspase-8-cleaved) Protein > 50ug; Carrier Free
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