Thermo Scientific™ POROS™ Oligo (dT)25 Affinity Resin

Beschreibung

POROS Oligo (dT)25 Affinity Resin is designed for the purification and isolation of mRNA from in vitro transcription (IVT) manufacturing processes. The resin effectively separates mRNA from components of the transcription reaction process, such as enzymes and plasmid DNA.

POROS Oligo (dT)25 Affinity Resin was developed to address the selectivity and capacity requirements for large-scale downstream purification of a range of mRNA constructs used in vaccine and gene therapy applications. Unlike alternate approaches, the resin selectively captures the mRNA via the polyadenylated (polyA) tail using simple salt and water purification steps.

Features of POROS Oligo (dT)25 Affinity Resin include:

• Easy mRNA purification from crude transcription mix to effectively remove plasmid DNA and other IVT components
• Simplified workflow helps to maximize efficiency, thereby reducing complexity of subsequent polish steps
• Excellent scalability
• Non-animal derived

POROS Oligo (dT)25 Affinity Resin allows for a robust platform and efficient purification process for a range of mRNA molecules and applications.

Pack sizes of 1 L and above come with a regulatory support package. Worldwide technical support is also available.

Main characteristics

• Matrix: cross-linked poly(styrene-divinylbenzene) POROS beads
• Average particle size: 50 μm
• Ligand: poly(dT) 25mer with proprietary linker
• Binding capacity: >2 mg mRNA per mL of resin
• Binding conditions: 0.5–1 M salt, common buffers, neutral pH
• Elution conditions: Low salt, common buffers, neutral pH
• Mechanical resistance: 100 bar (1450 psi; 10 MPa)
• Thermal stability: allows sample denaturing at 65°C if needed
• Formulation buffer: 18% (v/v) ethanol

Oligo (dT) ligands are manufactured using a synthetic manufacturing process that are free of animal components.