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Invitrogen™ Lyo-ready Bst DNA Polymerase

Invitrogen Lyo-ready Bst-DNA-Polymerase ist eine firmeneigene Mutation von Bst-DNA-Polymerase, einem großen Fragment, mit einer schnellen Reaktionsgeschwindigkeit, erhöhter Empfindlichkeit und erhöhter Toleranz gegenüber Inhibitoren.

65.00 CHF - 268.00 CHF

Spezifikation

Produkttyp Stand-alone enzyme
Zur Verwendung mit (Anwendung) LAMP, RT-LAMP
Polymerase Lyo-ready Bst DNA Polymerase
Optimale Reaktionstemperatur 65 °C
Versandbedingung Trockeneis
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Produkte 3
Produktcode Marke Konzentration Menge Preis Menge & Verfügbarkeit  
Produktcode Marke Konzentration Menge Preis Menge & Verfügbarkeit  
17815017
Unterlagen ansehen
Invitrogen™
A56655
6 U/μL 1200 U
65.00 CHF
1200 Einheiten
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17805217
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Invitrogen™
A56656
6 U/μL 6000 U
268.00 CHF
6000 Einheiten
Verfügbar ab: 12-12-2024
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Zum Warenkorb hinzufügen
 
17885207
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Invitrogen™
A56657
40 U/μL 1200 U
65.00 CHF
1200 Einheiten
Lager vorrätig
Sie sich ein, um den Lagerbestand zu sehen
Zum Warenkorb hinzufügen
 
Beschreibung

Beschreibung

Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).

Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).

Lyo-ready Bst DNA Polymerase possesses 5´→3´ DNA polymerase activity and lacks 5´→3´ exonuclease activity and 3´→5´ exonuclease activity. It is also a glycerol-free enzyme, enabling assay lyophilization and use in microfluidics-based technologies.

Lyo-ready Bst DNA Polymerase features
• Fast target detection in as little as 10 minutes
• Enhanced sensitivity down to 50 copies using optimized reaction buffer in (RT-)LAMP reaction
• Robust amplification, even from inhibitor-containing RNA or DNA samples
• Ability to optimize LAMP or RT-LAMP reactions to fit your needs

Applications
Lyo-ready Bst DNA Polymerase can be used for a wide variety of applications including:
• Loop-mediated isothermal amplification (LAMP)
• Whole genome amplification (WGA)
• Rolling circle amplification (RCA)
• Recombinase polymerase amplification (RPA)
• Helicase-dependent amplification (HDA)
• Exponential amplification reaction (EXPAR)

Notes
1. If performing real-time LAMP detection with SYTO 9 Green Fluorescent Nucleic Acid Stain, dilute the stock to 50 μM with nuclease-free water before every use.
2. SuperScript IV Reverse Transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor are recommended for reactions containing RNA targets.

Spezifikation

Spezifikation

Stand-alone enzyme
Lyo-ready Bst DNA Polymerase
Trockeneis
LAMP, RT-LAMP
65 °C
≤10 minutes
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

Dokumentation

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