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Invitrogen™ Lyo-ready Bst DNA Polymerase
Invitrogen Lyo-ready Bst-DNA-Polymerase ist eine firmeneigene Mutation von Bst-DNA-Polymerase, einem großen Fragment, mit einer schnellen Reaktionsgeschwindigkeit, erhöhter Empfindlichkeit und erhöhter Toleranz gegenüber Inhibitoren.
65.00 CHF - 268.00 CHF
Spezifikation
Produkttyp | Stand-alone enzyme |
---|---|
Zur Verwendung mit (Anwendung) | LAMP, RT-LAMP |
Polymerase | Lyo-ready Bst DNA Polymerase |
Optimale Reaktionstemperatur | 65 °C |
Versandbedingung | Trockeneis |
Produktcode | Marke | Konzentration | Menge | Preis | Menge & Verfügbarkeit | ||||
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Produktcode | Marke | Konzentration | Menge | Preis | Menge & Verfügbarkeit | ||||
17815017
|
Invitrogen™
A56655 |
6 U/μL | 1200 U |
65.00 CHF
1200 Einheiten |
Lager vorrätigLoggen Sie sich ein, um den Lagerbestand zu sehen
|
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17805217
|
Invitrogen™
A56656 |
6 U/μL | 6000 U |
268.00 CHF
6000 Einheiten |
Verfügbar ab: 27-11-2024
Loggen Sie sich ein, um den Lagerbestand zu sehen |
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17885207
|
Invitrogen™
A56657 |
40 U/μL | 1200 U |
65.00 CHF
1200 Einheiten |
Lager vorrätigLoggen Sie sich ein, um den Lagerbestand zu sehen
|
||||
Beschreibung
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).
Lyo-ready Bst DNA Polymerase possesses 5´→3´ DNA polymerase activity and lacks 5´→3´ exonuclease activity and 3´→5´ exonuclease activity. It is also a glycerol-free enzyme, enabling assay lyophilization and use in microfluidics-based technologies.
Lyo-ready Bst DNA Polymerase features
• Fast target detection in as little as 10 minutes
• Enhanced sensitivity down to 50 copies using optimized reaction buffer in (RT-)LAMP reaction
• Robust amplification, even from inhibitor-containing RNA or DNA samples
• Ability to optimize LAMP or RT-LAMP reactions to fit your needs
Applications
Lyo-ready Bst DNA Polymerase can be used for a wide variety of applications including:
• Loop-mediated isothermal amplification (LAMP)
• Whole genome amplification (WGA)
• Rolling circle amplification (RCA)
• Recombinase polymerase amplification (RPA)
• Helicase-dependent amplification (HDA)
• Exponential amplification reaction (EXPAR)
Notes
1. If performing real-time LAMP detection with SYTO 9 Green Fluorescent Nucleic Acid Stain, dilute the stock to 50 μM with nuclease-free water before every use.
2. SuperScript IV Reverse Transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor are recommended for reactions containing RNA targets.
Spezifikation
Stand-alone enzyme | |
Lyo-ready Bst DNA Polymerase | |
Trockeneis |
LAMP, RT-LAMP | |
65 °C | |
≤10 minutes |