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Invitrogen™ Lyo-ready Bst DNA Polymerase

Invitrogen Lyo-ready Bst-DNA-Polymerase ist eine firmeneigene Mutation von Bst-DNA-Polymerase, einem großen Fragment, mit einer schnellen Reaktionsgeschwindigkeit, erhöhter Empfindlichkeit und erhöhter Toleranz gegenüber Inhibitoren.

Marke:  Invitrogen™ A56656

214.40 CHF gültig bis 2024-12-20
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Artikelnummer. 17805217

  • 268.00 CHF / 6000 Einheiten
Verfügbar ab: 27-11-2024
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Beschreibung

Beschreibung

Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).

Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).

Lyo-ready Bst DNA Polymerase possesses 5´→3´ DNA polymerase activity and lacks 5´→3´ exonuclease activity and 3´→5´ exonuclease activity. It is also a glycerol-free enzyme, enabling assay lyophilization and use in microfluidics-based technologies.

Lyo-ready Bst DNA Polymerase features
• Fast target detection in as little as 10 minutes
• Enhanced sensitivity down to 50 copies using optimized reaction buffer in (RT-)LAMP reaction
• Robust amplification, even from inhibitor-containing RNA or DNA samples
• Ability to optimize LAMP or RT-LAMP reactions to fit your needs

Applications
Lyo-ready Bst DNA Polymerase can be used for a wide variety of applications including:
• Loop-mediated isothermal amplification (LAMP)
• Whole genome amplification (WGA)
• Rolling circle amplification (RCA)
• Recombinase polymerase amplification (RPA)
• Helicase-dependent amplification (HDA)
• Exponential amplification reaction (EXPAR)

Notes
1. If performing real-time LAMP detection with SYTO 9 Green Fluorescent Nucleic Acid Stain, dilute the stock to 50 μM with nuclease-free water before every use.
2. SuperScript IV Reverse Transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor are recommended for reactions containing RNA targets.

TRUSTED_SUSTAINABILITY
Spezifikation

Spezifikation

Stand-alone enzyme
Lyo-ready Bst DNA Polymerase

• Lyo-ready Bst DNA Polymerase (5 x 200 μL at 6 U/μL)
• 10X Bst Reaction Buffer (3 x 1 mL)
• 200 mM MgCl2 (1 mL)
• 5 M Betaine (3 x 1 mL)

Store at –15 to –25°C.

65 °C
6 U/μL
LAMP, RT-LAMP
6000 U
1000 Reaktionen
Trockeneis
≤10 minutes
Produktvorschläge

Produktvorschläge

Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

Dokumentation

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