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Gibco™ Human TGF-beta 1 Recombinant Protein
Recombinant Protein
Marke: Gibco™ PHG9202
Beschreibung
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This lyohpilized protein should be reconstituted in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C. Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C to -80°C for long term storage, preferably desiccated. Working aliquots stored with a carrier protein are stable for at least 12 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.
Spezifikation
P01137 | |
protein with no preservative | |
25 kDa | |
Sequential Chromatography | |
<0.1 ng/ μg | |
TGF beta-1 | |
Unconjugated | |
Recombinant | |
-20°C | |
Wet Ice | |
Growth Factors & Receptors | |
Lyophilized | |
Immunology, Neurobiology, Stem Cell Research, Bone Research, Cardiovascular Research, Oncology | |
>98% by SDS-PAGE |
Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot | |
7040 | |
Human TGF-beta 1 | |
250 μg | |
CED; cytokine; DPD1; LAP; Latency-associated peptide; prepro-transforming growth factor beta-1; regulatory protein; TGF b; TGF b 1; TGF beta; tgf beta 1; tgf beta1; TGF beta-1; TGF beta-1 protein; TGF β; TGF β 1; TGF β1; Tgfb; TGFB1; TGF-B1; Tgfb-1; TGFbeta; TGF-beta 1; TGF-beta 1 protein; TGFbeta1; TGF-beta1; TGF-beta-1; TGFβ1; Transforming growth factor; transforming growth factor b1; transforming growth factor beta; transforming growth factor beta 1; transforming growth factor beta-1; Transforming growth factor beta-1 proprotein; transforming growth factor beta-1 proprotein; transforming growth factor beta-1; transforming growth factor β1; transforming growth factor, beta 1; transforming growth factor, beta 1 (Camurati-Engelmann disease); transforming growth factor, beta-1; transforming growth factor-beta 1; transforming growth factor-beta I; transforming growth factor-beta-1; transforming growth factor-beta-1 precursor; unnamed protein product | |
ED50 ≤ 0.05 ng/mL; determined by the dose-dependent inhibition of mouse IL-4-dependent mouse HT-2 cell proliferation. | |
Human | |
Human TGF-beta 1 recombinant protein is a homodimer consisting of two disulfide-linked 112 amino acid subunits | |
<0.1 ng/μg | |
CHO cells | |
Recombinant, Ligand | |
TGFs (Transforming Growth Factors) | |
Gibco™ |
Zertifikate
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Chargennummer | Art des Zertifikats | Datum | Catalog Number |
---|---|---|---|
1584569A | Analysenzertifikat | 14/04/2017 | PHG9202 |
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