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Thermo Scientific™ Tango™ EDG1-bla U2OS Cells
The Tango™ EDG1-bla U2OS cells contain the human Endothelial Differentiation, Sphingolipid G-protein-coupled Receptor, 1 (EDG1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.
Marke: Thermo Scientific™ K1520
Beschreibung
The Tango™ EDG1-bla U2OS cells contain the human Endothelial Differentiation, Sphingolipid G-protein-coupled Receptor, 1 (EDG1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ EDG1-bla U2OS cells cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ EDG1-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.
Spezifikation
NM_001400.2 | |
Teilende Zellen | |
Nativer Schwanz | |
GPCRs | |
EDG1-bla U2OS-Zellen, EDG1-bla U2OS-Assay-Protokoll, Analysenzertifikat | |
Tango™ | |
BLA (Beta-Lactamase) | |
U2OS-Zelle | |
Tango™ |
Zellbasierte Beta-Arrestin-Rekrutierung | |
K1520 Tango™ EDG1-bla U2OS-Zellen (LiveBLAzer™-FRET B⁄G Lade-Kit, Lösung D und andere Materialien sind separat erforderlich; siehe Protokoll). Lieferumfang: • Tango™ EDG1-bla U2OS-Zellen • Tango™ EDG1-bla U2OS-Assay-Protokoll • Analysenzertifikat Lagerung: Die Tango™ EDG1-bla U2OS-Zellen werden auf Trockeneis geliefert und sollten sofort nach Erhalt in flüssigem Stickstoff gelagert werden. |
|
Fluoreszent | |
EDG1 | |
Auf Einzelzellen basierende Klone | |
2 x 106 Zellen | |
EDG1 | |
U2OS | |
GPCRs |
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.