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R&D Systems™ Recombinant Mouse Tryptase beta-1/Mcpt7 Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity
Marke: R&D Systems™ 1937-SE-020
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Beschreibung
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >75 pmol/min/μg, as measured under the described conditions.Spezifikation
| Q02844 | |
| Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2. | |
| M.W. (Observed): 36 kDa and 37 kDa, reducing conditions; M.W. (theoretical): 28.8 kDa | |
| 20 μg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening. | |
| <1.0 EU / 1 μg of the protein by the LAL method. | |
| Unconjugated | |
| >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| Enzyme Activity | |
| 17230 | |
| Tryptase beta-1/MCP-7/Mcpt7 | |
| Mouse myeloma cell line, NS0-derived mouse Tryptase beta-1/MCP-7 protein Ile29-Phe273, with an C-terminal 10-His tag | |
| Mature form | |
| Tpsab1 | |
| Recombinant | |
| Tryptase beta-1/MCP-7/Mcpt7 |
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