Thermo Scientific™ Pierce™ NeutrAvidin™ Coated High Capacity Plates

Beschreibung

Biotin-Bindungsplatten eignen sich ideal für Anwendungen bei direkter Adsorption von Polystyrol-denaturierten Antikörpern oder dem Zielmolekül. Thermo Scientific NeutrAvidin Protein ist Avidin, das deglykosyliert wurde, um eine unspezifische Lektin-Bindung zu verhindern. Mit nahezu neutralem IEP (6,3) führt dies in bestimmten Anwendungen zu geringeren unspezifischen Wechselwirkungen im Vergleich zu Streptavidin. Dem NeutrAvidin Protein fehlt die bakterielle RYD-Sequenz auf Streptavidin, was eine unspezifische Bindung aufgrund der RGD-Bindungsdomäne von Adhäsionsrezeptoren in einer Vielzahl von Zellen eliminiert.

Thermo Scientific NeutrAvidin™ Coated High Capacity Plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Thermo Scientific Pierce Coated Plates include:

• Unique plate-coating technology – results in high loading and binding capacity
• NeutrAvidin Protein – coated plates have up to twice the binding capacity of typical plates
• Robust and sensitive – NeutrAvidin Protein yields less nonspecific binding for improved signal-to-noise ratios
• Broader dynamic range – extends the quantitative range so there is less need for dilutions
• Save time – pre-blocked plates to reduce the number of assay steps
• Flexible assay formats – choose from 96- and 384-well formats in a variety of colors

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

For detection of low concentrations (ng/mL) of biotinylated antibodies and other proteins, check out

Pierce™ NeutrAvidin™ Coated High Sensitivity Plates, Clear, 8-Well Strip