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Thermo Scientific™ GeneBLAzer™ PPARδ-UAS-bla HEK 293T Cells
PPAR delta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-delta (PPAR delta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.
Marke: Thermo Scientific™ K1690
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Beschreibung
PPAR delta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-delta (PPAR delta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-PPAR delta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. PPAR delta-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of L-165041. In addition, PPAR delta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
Spezifikation
| Estriol, Estron, Genistein, Diethylstibestrol, Ethinylestradiol | |
| Zellbasiertes Beta-Lactamase-Reportergen | |
| Flüssigstickstoff | |
| Primärer Agonist: 17-β-Östradiol | |
| Human | |
| PPAR delta-UAS-bla HEK 293T-Zellen (K1244) | |
| 17-β-Östradiol | |
| 2 x 106 Zellen | |
| BLA (Beta-Lactamase) | |
| Teilende Zellen |
| RU486+1 nM E2, 4-OH-Tam+1 nM E2, 4-OH-Tamoxifen, Tamoxifen, Tam+1 nM E2, RU486 | |
| Teilende Zellen | |
| Fluoreszent | |
| Kernrezeptoren, nukleäre Rezeptoren | |
| Kernrezeptoren, nukleäre Rezeptoren | |
| 2 x 106 | |
| GeneBLAzer™ | |
| HEK 293T-Zelle | |
| PPAR delta, NR1C2, PPARD |
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.