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Thermo Scientific™ GeneBLAzer™ OPRL1-Gqi5-NFAT-bla FreeStyle™ 293F Cells
The GeneBLAzer™ OPRL1-Gqi5-NFAT-bla FreeStyle™ 293F Cell Line contains the human opioid/opiate receptor-like-1 receptor (OPRL1) stably integrated into the GeneBLAzer™ Gqi5-NFAT-bla FreeStyle™ 293F Cell Line.
Marke: Thermo Scientific™ K1714
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Beschreibung
The GeneBLAzer™ OPRL1-Gqi5-NFAT-bla FreeStyle™ 293F Cell Line contains the human opioid/opiate receptor-like-1 receptor (OPRL1) stably integrated into the GeneBLAzer™ Gqi5-NFAT-bla FreeStyle™ 293F Cell Line. GeneBLAzer™ Gqi5-NFAT-bla FreeStyle™ 293F cells (Cat. No. K1539) contain the chimeric Gqi5 G protein and the beta-lactamase reporter gene under control of the nuclear factor of activated T cells (NFAT) response element.
OPRL1-Gqi5-NFAT-bla FreeStyle™ 293F cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time, and functionally validated for Z-factor and EC50 concentrations of orphanin FQ/nociceptin. These data are found in the Validation & Assay Performance Summary.
Orphanin FQ/nociceptin and OPRL1 have been implicated in various physiological functions. The most defined role is their involvement in the mediation of pain in the central nervous system and their possible roles in opiate tolerance, opiate dependence and withdrawal, and the ability to cope with stress and anxiety. Through the use of antagonists, antisense oligos, and transgenic knockout mutants, OPRL1 has been implicated in additional systems including learning, memory, and reproduction.
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Spezifikation
| NM_000913 | |
| Teilende Zellen | |
| Fluoreszent | |
| OPRL1 | |
| Auf Einzelzellen basierende Klone | |
| 2 x 106 Zellen | |
| OPRL1 | |
| FreeStyle 293-F-Zellen | |
| GPCR |
| Zellbasiertes Beta-Lactamase-Reportergen | |
| Inhalt: K1714 GeneBLAzer™ OPRL1-Gqi5-NFAT-bla Freestyle 293F-Zellen (LiveBLAzer™ FRET B⁄G Ladekit, Lösung D und andere Materialien sind separat erhältlich; siehe Protokoll). Enthält: • GeneBLAzer™ OPRL1-Gqi5-NFAT-bla Freestyle 293F-Zellen • GeneBLAzer™ OPRL1-Gqi5-NFAT-bla Freestyle 293F Assay-Protokoll • Analysenzertifikat Lagerung: Die GeneBLAzer™ OPRL1-Gqi5-NFAT-bla Freestyle 293F-Zellen werden auf Trockeneis geliefert und sollten sofort nach Erhalt in flüssigem Stickstoff gelagert werden. |
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| FRET | |
| GeneBLAzer™ OPRL1-Gqi5-NFAT-bla Freestyle 293F cells, GeneBLAzer™ OPRL1-Gqi5-NFAT-bla Freestyle 293F Assay | |
| GeneBLAzer™ | |
| BLA (Beta-Lactamase) | |
| 293F-Zelle | |
| GeneBLAzer™ |
For Research Use Only. Not for use in diagnostic procedures.