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Thermo Scientific™ EquiPhi29™ DNA Amplification Kit

The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).

355.00 CHF - 1480.00 CHF

Spezifikation

Produkttyp DNA Amplification Kit
Form Flüssig
Zur Verwendung mit (Anwendung) RCA, MDA-WGA
Polymerase EquiPhi29 DNA Polymerase
Forschungskategorie Nucleic Acid Analysis, In Vitro Transcription, Cell-free Protein Expression, In Situ Sequencing, SNP Detection
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Produkte 2
Produktcode Marke Menge Preis Menge & Verfügbarkeit  
Produktcode Marke Menge Preis Menge & Verfügbarkeit  
18094070
Unterlagen ansehen Aktionsdetails
Thermo Scientific™
A65393
100 Reactions
355.00 CHF
100 Reaktionen
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18084100
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Thermo Scientific™
A65394
500 Reactions
1480.00 CHF
500 Reaktionen
Verfügbar ab: 27-11-2024
Sie sich ein, um den Lagerbestand zu sehen
Zum Warenkorb hinzufügen
 
Beschreibung

Beschreibung

The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).

Features of the EquiPhi29 DNA Amplification Kit include:
• Fast DNA amplification in less than two hours
• Sensitivity from 1 fg of input DNA
• Maximal yield of 17 µg amplified DNA
• Compatibility with different types of sample input material (e.g., purified DNA, liquid media culture, agar plate colonies)
• Compatibility with various downstream applications such as DNA sequencing (Sanger sequencing, next-generation sequencing), digestion with restriction enzymes, cell-free DNA enrichment, and cell-free protein expression
• All required components for DNA amplification in RCA or MDA-WGA reactions

The kit contains EquPhi29 DNA Polymerase, a proprietary phi29 DNA polymerase mutant that possesses strong strand displacement activity and enables high thermostability, reaction speed, and product yield and low amplification bias. Also included is an optimized reaction buffer, exo-resistant random primer, pyrophosphatase, and dNTPs. The addition of pyrophosphatase to the reaction mixture enhances DNA amplification and facilitates sample handling.

Spezifikation

Spezifikation

DNA Amplification Kit
RCA, MDA-WGA
Nucleic Acid Analysis, In Vitro Transcription, Cell-free Protein Expression, In Situ Sequencing, SNP Detection
42 °C
Up to 2 hours
Flüssig
EquiPhi29 DNA Polymerase
Kit
Trockeneis
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

Dokumentation

Product Certifications

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.