Invitrogen™ Dynabeads™ Biotin Binder

Beschreibung

Dynabeads Biotin Binder contains 2.8 μm superparamagnetic beads that bind biotinylated antibodies (or other biotinylated ligands), allowing you to isolate cells from any species (e.g., human, mouse, rat, non-human primates, and cell lines). Advantages of Dynabeads Biotin Binder:

• Works with any cell type, from any sample and from any species
• Fast and efficient depletion or positive isolation of any target cells for molecular downstream assays using a biotinylated primary antibody
• Obtain pure and untouched cells using a mixture of biotinylated antibodies to deplete unwanted cells

Adaptable for various isolation strategies

The biotinylated primary antibody may be added to the cell sample (indirect technique) or pre-coated onto the Dynabeads Biotin Binder (direct technique). Regardless of which technique is used, when the cell sample and the Dynabeads Biotin Binder are mixed, the beads bind to the target cells. Placing the sample on a magnet separates the bead-bound cells from the rest of the sample. One or more biotinylated antibodies may be used to either deplete the sample of unwanted cells (negative isolation) or to capture specific cells for downstream experiments (positive isolation). For isolation of bead-free cells (e.g., for flow cytometry), we recommend using your biotinylated antibody in combination with the CELLection Biotin Binder Kit.

Useful for many sample types

Using Dynabeads Biotin Binder, you can isolate cells basically from any species, and from many starting samples such as whole blood, cord blood, peripheral blood mononuclear cells (PBMC), buffy coat, mouse spleen/lymph nodes, and tissue digests.

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