Applied Biosystems™ Custom TaqMan™ SNP Genotyping Assay, non-human

Beschreibung

Erstellen Sie Assays, indem Sie vertrauliche Zielsequenzen mit dem anwenderspezifischen TaqMan-Assay-Design-Tool an Applied Biosystem's sichere Assay-Design-Pipeline übermitteln. Diese Pipeline hat Millionen von Assay-Designs generiert, indem sie heuristische Regeln verwendet hat, die sowohl aus Herstellungs- als auch aus Assay-Leistungsdaten abgeleitet wurden. Diese Assays verwenden TaqMan Sonden, die in der kleinen Furche binden (Minor Groove Binding, MGB) für überlegene allelische Unterscheidung, verbesserte Signal-Rausch-Verhältnisse und Designflexibilität. Alle humanen SNP-Genotypisierungsassays werden funktionell auf 20 einzigartige DNA-Proben getestet, um die Assay-Amplifikation und Probenclustering zu gewährleisten. Diese kundenspezifischen Assays werden entwickelt, synthetisiert, formuliert, optimiert und Qualitätskontrolltests unterzogen. Sie eignen sich ideal für Genotypisierungsanwendungen wie Screening, Assoziation, Kandidatenregion, Kandidatengen oder Feinkartierungsstudien.

Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5´‑nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNA samples. Each assay enables genotyping of individuals for a single nucleotide polymorphism (SNP) and consists of two sequence-specific primers and two TaqMan minor groove binder (MGB) probes with non-fluorescent quenchers (NFQ). One probe is labeled with VIC dye to detect the Allele 1 sequence; the second probe is labeled with FAM dye to detect the Allele 2 sequence.

Custom TaqMan SNP Genotyping Assays can be easily designed at no extra charge by submitting target sequences confidentially to our secure Custom Assay Design Tool. The tool can generate assay designs targeting any SNP in any organism, offering maximum flexibility to meet your research needs.

Benefits:
• Proven—gold-standard TaqMan chemistry and robust assay designs deliver accurate, reproducible, and reliable results
• Easy—convenient single-tube format and simple workflow provide an easy path to trusted results; no optimization required
• Flexible—obtain assay designs for any SNP in any organism using our secure Custom Assay Design Tool, at no extra charge
• Tested—all custom assays are quality-control tested for synthesis accuracy and formulation completeness

Approximate ship time
4–6 days in North America and 6–10 days in Europe

The free Custom Assay Design Tool can generate assays for the detection of SNPs, MNPs, and indels of up to six bases. These custom assays are designed, synthesized, formulated, optimized, and quality control tested.

TaqMan SNP Genotyping Assays require only three reaction components for PCR: purified genomic DNA (1–20 ng), the assay solution, and TaqMan Genotyping Master Mix (or another compatible master mix) (sold separately).

All assay designs are the product of our industry-leading bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.

Recommended master mix (sold separately): TaqMan Genotyping Master Mix (Cat. No. 4371355)