Invitrogen™ Human Apolipoprotein C3 ELISA Kit

Beschreibung

The Human Apolipoprotein C3 (Hu ApoC3) ELISA quantitates Hu ApoC3 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ApoC3. Principle of the method The Human ApoC3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Apolipoprotein C3 (Apo C3) wird hauptsächlich in der Leber produziert und zirkuliert im Plasma in Kombination mit Apo B-Lipoprotein und Lipoproteinen hoher Dichte. Apo C3 wirkt als Antagonist zu Apo C2 und Apo E, um die Lipolyse durch das Enzym Lipoproteinlipase zu verhindern und auch die Freisetzung von Apo B. Das Gen, das für Apo C3 codiert, findet sich auf Chromosom 11.