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ZO-1 Polyclonal Antibody, Invitrogen™
Rabbit Polyclonal Antibody
Marke: Invitrogen 617300
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Beschreibung
61-7300 has been successfully used in Western blot, Immunoprecipitation, Immunofluorescence, ELISA and Immunohistochemistry. To achieve appropriate staining results on formalin-fixed, paraffin embedded tissue sections, protease Type XIV (Actinase E) pretreatment is required. The recommended dilution and incubation for protease is 1-2 mg/mL at 37°C for 10 minutes. Other enzyme and Heat Induced Epitope Retrieval pre-treatment methods may not be suitable for this particular antibody. Frozen sections have been successfully stained after fixation with acetone at 4°C.
ZO-1 Polyclonal antibody specifically detects ZO-1 in Canine, Guinea Pig, Human, Mouse, Rat samples. It is validated for ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot.
Spezifikation
| ZO-1 | |
| Polyclonal | |
| Unconjugated | |
| TJP1 | |
| DKFZp686M05161; I79_005485; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); tight junction protein ZO-1; Tjp1; zo1; ZO-1; ZO-1 MDCK; zona occludens 1; zona occludens protein 1; zonula occludens 1; zonula occludens 1 protein; zonula occludens protein 1 | |
| Rabbit | |
| Antigen affinity chromatography | |
| RUO | |
| 100718142, 21872, 292994, 403752, 7082 | |
| -20°C | |
| Liquid |
| ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot | |
| 0.25 mg/mL | |
| PBS with 0.1% sodium azide; pH 7.4 | |
| O97758, P39447, Q07157 | |
| TJP1 | |
| A 69 kD fusion protein(1) corresponding to amino acids 463-1109 of human ZO-1 cDNA.(2) This sequence lies N-terminal to the 80 amino acid region (the alpha-motif) present in the a+-isoform but absent in the a- isoform due to alternative splicing. | |
| 100 μg | |
| Primary | |
| Canine, Guinea Pig, Human, Mouse, Rat | |
| Antibody | |
| IgG |
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