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Thermo Scientific™ Tango™ EDG8-bla U2OS Cells
The Tango™ EDG8-bla U2OS cells contain the human Endothelial Differentiation Gene 8 (EDG8) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.
Marke: Thermo Scientific™ K1518
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Beschreibung
The Tango™ EDG8-bla U2OS cells contain the human Endothelial Differentiation Gene 8 (EDG8) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ EDG8-bla U2OS cells are functionally validated for Z' and EC50 concentrations of S1P. In addition, Tango™ EDG8-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.
For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
Spezifikation
| NM_030760.3 | |
| Teilende Zellen | |
| Schwanz von AVPR2 GPCR | |
| FRET | |
| EDG8-bla U2OS-Zellen, EDG8-bla U2OS-Assay-Protokoll, Analysenzertifikat | |
| GeneBLAzer™, Tango™ | |
| Endpunkt | |
| EDG8 | |
| U2OS | |
| GPCRs |
| Zellbasierte Beta-Arrestin-Rekrutierung | |
| Inhalt: K1518 GeneBLAzer™ EDG8-bla U2OS Zellen (LiveBLAzer™-FRET B⁄G Ladekit, Lösung D und andere Materialien sind separat erhältlich; siehe Protokoll). Lieferumfang: • Tango™ EDG8-bla U2OS-Zellen • Tango™ EDG8-bla U2OS Assay-Protokoll • Analysenzertifikat Lagerung: Die Tango™ EDG8-bla U2OS-Zellen werden auf Trockeneis geliefert und sollten sofort nach Erhalt in flüssigem Stickstoff gelagert werden. |
|
| Fluoreszent | |
| EDG8 | |
| Pool aus stabilen Zellen | |
| 8 x 106 Zellen | |
| BLA (Beta-Lactamase) | |
| U2OS-Zelle | |
| Tango™ |
For Research Use Only. Not for use in diagnostic procedures.