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Invitrogen™ SuperScript IV UniPrime One-Step RT-PCR System

Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.

375.00 CHF - 5000.00 CHF

Spezifikation

Nachweisverfahren Gel Electrophoresis
Format 1-step RT-PCR Kit
GC-Rich PCR Performance Hoch
PCR-Methode 1-step RT-PCR
Verfahren 1-step RT-PCR
Mehr Spezifikationen ansehen

Produkte 4
Produktcode Marke Beschreibung Anzahl Reaktionen Preis Menge & Verfügbarkeit  
Produktcode Marke Beschreibung Anzahl Reaktionen Preis Menge & Verfügbarkeit  
17871544
Unterlagen ansehen Aktionsdetails
Invitrogen™
12596025
Dye-free 25 x 50 μL Reactions
375.00 CHF
25 Reaktionen
Lager vorrätig
Sie sich ein, um den Lagerbestand zu sehen
Zum Warenkorb hinzufügen
 
17881544
Unterlagen ansehen Aktionsdetails
Invitrogen™
12597025
Colored 25 x 50 μL Reactions
375.00 CHF
25 Reaktionen
Lager vorrätig
Sie sich ein, um den Lagerbestand zu sehen
Zum Warenkorb hinzufügen
 
17804584
Unterlagen ansehen Aktionsdetails
Invitrogen™
12596500
Dye-free 500 x 50 μL Reactions
5000.00 CHF
500 Reaktionen
Lager vorrätig
Sie sich ein, um den Lagerbestand zu sehen
Zum Warenkorb hinzufügen
 
17814604
Unterlagen ansehen Aktionsdetails
Invitrogen™
12597500
Colored 500 x 50 μL Reactions
5000.00 CHF
500 Reaktionen
Lager vorrätig
Sie sich ein, um den Lagerbestand zu sehen
Zum Warenkorb hinzufügen
 
Beschreibung

Beschreibung

Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube. It combines the high-processivity SuperScript IV Reverse Transcriptase (RT) with a novel UniPrime RT-PCR Master Mix to provide easier use and unmatched performance when compared to other one-step RT-PCR kits.

The Invitrogen SuperScript IV UniPrime One-Step RT-PCR System offers a convenient one-step process  for performing fast cDNA synthesis and PCR amplification in a single tube. It combines high-processivity SuperScript IV Reverse Transcriptase (RT) with a novel UniPrime RT-PCR master mix for easier use and unmatched  performance compared to other one-step RT-PCR kits.

Highlights
Simplified workflow—universal primer annealing at 60°C decreases optimization time
Easy pipetting—colored buffers for visual tracking of reaction setup and direct sample loading on gels
Suitable for automation—two-phase hot-start activation mechanism for room temperature setup and benchtop stability for up to 24 hours
Superior performance—high yields and reliable target detection even with challenging RNA samples

Combination of superior enzymes
The SuperScript IV UniPrime One-Step RT-PCR System utilizes a combination of superior enzymes. The SuperScript IV Reverse Transcriptase mix helps produce an efficient reverse transcription step through its high thermostability, processivity, and ability to synthesize cDNA from variety of RNA samples, while its high sensitivity helps ensure reliable target detection even when RNA input is limited (down to 0.01 pg RNA).

The 2X UniPrime RT-PCR master mix contains modified Platinum SuperFi II DNA Polymerase, which delivers high specificity and high yields and is ideally suited for applications that require sequence accuracy. This novel master mix formulation enables universal primer annealing at 60°C for most primer pairs designed following the general design rules and helps minimize optimization steps, save time, and avoid mistakes in RT-PCR runs.

Two-phase hot start
The innovative two-phase hot-start mechanism helps deliver high specificity and yield in one-step RT-PCR through temporal separation of the activities of the reverse transcriptase and DNA polymerase. This not only allows reaction setup at room temperature, but also helps ensure the stability of assembled reactions for up to 24 hours for targets up to 3 kb and for up to 4h for targets >3 kb.

Colored format
The SuperScript IV UniPrime One-Step RT-PCR System is availabe in a standard dye-free format or colored format containing tracking dyes. When the red tracking dye in the SuperScript IV RT mix and the blue tracking dye in the UniPrime RT-PCR master mix are combined during reaction setup, the final reaction mix turns to purple. This allows for simple visual tracking of reaction setup and helps prevent pipetting errors. The final reaction product can also be directly loaded on agarose gels for a more streamlined workflow.

Spezifikation

Spezifikation

Gel Electrophoresis
Hoch
1-step RT-PCR
50°C to 65°C
Built-in Hot Start
Reverse Transkription
RNA
1-step RT-PCR Kit
1-step RT-PCR
Yes
SuperScript IV
Master Mix
Bis 13 kb
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

Dokumentation

Product Certifications

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.