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R&D Systems™ Recombinant Human BID (Caspase-8-cleaved) Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity
Marke: R&D Systems™ 882-B8-050
464.78 CHF gültig bis 2024-12-16
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Beschreibung
Measured by its ability to induce cytochrome c release from isolated mouse liver mitochondria using the Rat/Mouse Cytochrome c Quantikine ELISA (Catalog # MCTC0 ) to quantify cytochrome c. The typical EC50 for cytochrome c releasing activity is <50 nM. The EC50 for the desired application should be determined. Uncleaved Recombinant Human BID (Catalog # 846-BD ) is available.Spezifikation
| P55957 | |
| Supplied as a 0.2 μm filtered solution in HEPES-KOH and KCl. | |
| M.W. (Observed): 9 kDa &14 kDa, reducing conditions; M.W. (theoretical): 7 kDa (N-terminal fragment) & 15 kDa (C-terminal fragment) | |
| 50 μg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening. | |
| Unconjugated | |
| >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| Enzyme Activity | |
| 637 | |
| BID Caspase-8-cleaved | |
| E. coli-derived human BID protein Met1-Asp195, protein was purified, cleaved by Caspase-8 and further purified | |
| BID | |
| Recombinant | |
| BID |