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R&D Systems™ Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity, Binding Activity
Marke: R&D Systems™ 1246-4L-025
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Beschreibung
Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.008-0.05 μg/mL in the presence of a Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050 ). Measured by its ability to bind Recombinant Mouse 4-1BB/TNFRSF9/CD137 Fc Chimera (Catalog # 937-4B ) in a functional ELISA.Spezifikation
| P41274 | |
| Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | |
| M.W. (Observed): 41-44 kDa, reducing conditions; M.W. (theoretical): 25.7 kDa | |
| Mouse myeloma cell line, NS0-derived mouse 4-1 BB Ligand/TNFSF9 protein Met 10-His tag GGGSGGGSGGGSIEGR Mouse 4-1 BB Ligand (Arg104-Glu309) (N-terminus) Accession # P41274 (C-terminus) | |
| <1.0 EU / 1 μg of the protein by the LAL method. | |
| 4-1BB Ligand/TNFSF9 | |
| Mouse | |
| Reconstitute at 100 μg/mL in sterile PBS containing (at least 0.1% human or bovine serum albumin.) | |
| 4-1 BB Ligand/TNFSF9 |
| Bioactivity, Binding Activity | |
| 8744 | |
| 25 μg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution. | |
| Tnfsf9 | |
| Unconjugated | |
| Recombinant | |
| >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
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