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R&D Systems™ Bacterial Thermolysin Protein, CF
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity
Marke: R&D Systems™ 3097-ZN-020
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Beschreibung
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005 ). The specific activity is >12,000 pmol/min/μg, as measured with 10 μM ES005 and 2 ng enzyme in 100 μL of 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij 35, pH 7.5.Spezifikation
| Enzyme Activity | |
| M.W. (Observed): 36 kDa, reducing conditions | |
| 20 μg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening. | |
| Unconjugated | |
| Thermolysin |
| Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol. | |
| Thermolysin | |
| Geobacillus stearothermophilus -derived Thermolysin protein | |
| <1.0 EU / 1 μg of the protein by the LAL method. | |
| >70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining. |
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