Invitrogen™ Proteinase K Recombinant Protein

Beschreibung

Proteinase K (E.C.3.4.21.64) is an extracellular serine endoproteinase produced by Tritirachinm album Limber. Proteinase K is active in a wide range of temperatures and buffers with optimal activity between 20 and 6°C and a pH between 7.5 and 12.0. Activity is stimulated when up to 2% SDS or up to 4 M urea are included in the reaction. Calcium is important for thermostability of Proteinase K but it is not required for catalysis, therefore Proteinase K is also active in buffers containing chelating agents such as EDTA.

• Purity: > 95% by SDS-Page
• Protein Length/ AA Sequence: full length Proteinase K protein
• Biological Activity: (Measured by its digestion activity. The activity is >30U/mg. One unit will digest Casein at 37°C (pH 7.5) per minute to produce equal absorbance as 1.0 μmol of L-tyrosine.
• Animal Origin Free: Yes No DNase contamination detected by digestion of lambda DNA at 37°C for 6 hours. No RNases contamination detected by digestion of lambda DNA at 25°C for 16 hours. Host genomic DNA/RNA is no residual detected by PCR. Storage: Store the lyophilized protein at 4°C for up to 1 year and less than -20°C for long term. Lyophilized powder is recommended to dissolve in in 20mM Tris-Hcl, 30mM NaCl, 50% Glycerol, 5mM CaCl2, pH 7.2-7.4. After reconstitution, the protein solution is stable at -20°C for 1 year, at 2-8°C for up to 6 months.