PMCA1 ATPase Polyclonal Antibody, Invitrogen™

Beschreibung

PA1-914 detects calcium pump of the plasma membrane 1a (PMCA1a ATPase) and 1b from rat, human and porcine tissues. PA1-914 has been successfully used in Western blot, immunohistochemistry (paraffin and frozen), and ELISA procedures. By Western blot, this antibody detects ∽134 kDa and ∽130 kDa proteins representing PMCA1a and 1b ATPase from rat, human and porcine samples. The PA1-914 immunogen is a synthetic peptide corresponding to the residues A(5) N N S V A Y S G V K N S I K E A N(22) of rat PMCA1 ATPase.

The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/potassium pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin.