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Thermo Scientific™ Maxima H Minus Reverse Transcriptase Promotion
A highly thermostable, high processivity reverse transcriptase lacking RNase H activity
Marke: Thermo Scientific™ EP0751
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Beschreibung
- Thermostable - 90% active after incubation at 50°C for 60 minutes in a reaction mixture.
- Active up to 65°C
- High yields of full-length cDNA up to 20 kb
- High sensitivity - reproducible cDNA synthesis from a wide range of starting total RNA amounts (1 pg to 5 μg)
- Efficient - complete cDNA synthesis in 15 to 30 minutes
- Increased resistance to common reaction inhibitors
- Incorporates modified nucleotides
Spezifikation
| 250 mM Tris-HCl buffer (pH 8.3 at 25°C), 375 mM KCl, 15 mM MgCl2, 50 mM DTT | |
| 2000 U | |
| 5X |
| -20 °C | |
| cDNA Synthesis, RT-PCR, Genexpression |