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Thermo Scientific™ GeneBLAzer™ TRβ-UAS-bla HEK 293T-Zellen
TR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Thyroid hormone receptor beta(TR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UASbla HEK 293T cell line.
Marke: Thermo Scientific™ K1686
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Beschreibung
TR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Thyroid hormone receptor beta(TR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UASbla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a betalactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-TR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. TR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of T3 Thryoid hormone. In addition, TR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
Spezifikation
| 9-cis-Retinsäure, AM580 | |
| Zellbasiertes Beta-Lactamase-Reportergen | |
| -80 °C/flüssiger Stickstoff | |
| GeneBLAzer™ TRβ-UAS-bla HEK 293T-Zellen, 2 x 106 Zellen, ∽2,000,000 Zellen/Fläschchen bei 2,000,000 Zellen/ml Zellmenge, Fluoreszenznachweismethode, Zellkern-Reporter Druggable Target, BLA (Beta-Lactamase)-Reporter, adhärente Wachstumseigenschaften, Lagerung in Flüssigstickstoff/–80 °C | |
| Human | |
| TR beta-UAS-bla HEK 293 T-Zellen (K1248) | |
| All-trans-Retinsäure (ATRA) | |
| 2 x 106 Zellen | |
| 293T (HEK) | |
| T3RB, TR Beta, Thrb, NR1A2 |
| RP-41-5253 | |
| Teilende Zellen | |
| Fluoreszent | |
| Pharmazie und Biopharmazie, Arzneimittelforschung und -entwicklung, Ziel- und Verlaufserkennung und -validierung, G-Protein-gekoppelte Rezeptorbiologie, zellbasierte GPCR-Reporter-Assays | |
| Kernrezeptoren, nukleäre Rezeptoren | |
| 2 x 106 Zellen | |
| GeneBLAzer™ | |
| HEK 293T-Zelle | |
| BLA (Beta-Lactamase) | |
| GeneBLAzer™ TRβ-UAS-bla HEK 293T-Zellen |
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.