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Thermo Scientific™ GeneBLAzer™ TR-alpha DA Assay-Kit
GeneBLAzer™ TR alpha DA (Division Arrested) cells and TR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Thyroid hormone receptor alpha(TR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UAS-bla HEK 293T cell line.
Marke: Thermo Scientific™ K1385
Beschreibung
GeneBLAzer™ TR alpha DA (Division Arrested) cells and TR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Thyroid hormone receptor alpha(TR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UAS-bla HEK 293T cell line. GeneBLAzer™ UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-TR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both TR alpha DA cells and TR alpha-UASbla HEK 293T 293 cells are functionally validated for Z' and EC50 concentrations of T3 Thryoid hormone. In addition, TR alpha-UAS-bla HEK 293T 293 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
Spezifikation
Zellbasiertes Beta-Lactamase-Reportergen | |
Versand: Trockeneisenlagerung: -80 °C/Flüssigstickstoff. Enthält ausreichend DA (Division Arrested)-Zellen und Substrat für den Assay einer 384-Well-Platte einschließlich TR alpha HEK 293T DA-Zellen (K1385A)LiveBLAzer™-FRET B/G Lade-Kit, 70 μg | |
Fluoreszent | |
7067 | |
TR alpha HEK 293T DA-Zellen (K1385A) LiveBLAzer™-FRET B/G Ladekit, 70 μg | |
1 Platte | |
293T (HEK) | |
TR-alpha DA Assay-Kit | |
GeneBLAzer™ |
Zellen mit arretierter Teilung | |
GeneBLAzer™TR Alpha DA-Assay Kit, 1 Platte, ∽4,800,000 Zellen/Fläschchen bei 4,800,000 Zellen/ml Zellmenge, 293T (HEK) Zelllinie, Fluoreszenznachweisverfahren, Zellkern-Reporter Druggable Target, BLA (Beta-Lactamase)-Reporter, dhärente Wachstumseigenschaften, Lagerung in Flüssigstickstoff/–80 °C | |
Zelluläre Bildgebung, Pharmzie und Biopharmzie, Ziel- und Verlaufserkennung und -validierung, zielgerichtete adme/tox-Assays, Zellanalyse, zellbasierte Reporterassays, zellbasierte und Lactamase-Assays, Arzneimittelforschung und -entwicklung, Kernrezeptorbiologie, zellkernbasierte Rezeptor-Reporter-Assays, zielgerichtete ADME/tox-Assays, zelluläre adme-Assays, Zellkern-Reporter-adme-Assays | |
TRα | |
GeneBLAzer™ | |
BLA (Beta-Lactamase) | |
THRA, TR alpha, NR1A1 | |
293T (HEK) | |
Kernrezeptoren |
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.