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Thermo Scientific™ GeneBLAzer™ RAR-gamma DA Assay Kit
GeneBLAzer™RAR gamma DA (Division Arrested) cells and RAR gamma-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor gamma (RAR gamma) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.
Marke: Thermo Scientific™ K1559
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Beschreibung
GeneBLAzer™RAR gamma DA (Division Arrested) cells and RAR gamma-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor gamma (RAR gamma) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RAR gamma (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both RAR gamma DA cells and RAR gamma-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of all-trans retinoic acid (ATRA). In addition, RAR gamma-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.
Spezifikation
| 4-Hydroxytamoxifen, Chlordan, Toxaphen | |
| Zellen mit arretierter Teilung | |
| GeneBLAzer™ RAR Gamma DA-Assay Kit, 1 Platte, ∽2,400,000 Zellen/Fläschchen bei Zellen/ml Zellmenge, 293T (HEK)-Zelllinie, Fluoreszenznachweisverfahren, Zellkern-Reporter Druggable Target, BLA (Beta-Lactamase)-Reporter, Lagerung in Flüssigstickstoff | |
| Pharmazie und Biopharmazie, target-basierte ADME-/tox-Assays, Arzneimittelforschung und -entwicklung, Ziel- und Verlaufserkennung und -validierung, G-Protein-gekoppelte Rezeptorbiologie, zellbasierte GPCR-Reporter-Assays | |
| RAR-gamma HEK 293T DA-Zellen, LiveBLAzer™-FRET B/G Ladekit, 70 μg | |
| 1 Platte | |
| 293T (HEK) | |
| RAR-gamma DA Assay-Kit | |
| GeneBLAzer™ |
| Zellbasiertes Beta-Lactamase-Reportergen | |
| Versand: Trockeneisenlagerung: -80 °C/Flüssigstickstoff Ausreichend „Division Arrested“-Zellen und Substrat, für den Assay einer 384-Well-Platte. Lieferumfang: RAR-gamma HEK 293T DA-Zellen (K1559A)LiveBLAzer™-FRET B/G Lade-Kit, 70 μg | |
| Fluoreszent | |
| RARγ | |
| XCT790 | |
| BLA (Beta-Lactamase) | |
| RARG, RAR-gamma, NR1B3 | |
| 293T (HEK) | |
| Kernrezeptoren |
For Research Use Only. Not for use in diagnostic procedures.