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Thermo Scientific™ GeneBLAzer™ PPAR gamma DA Assay-Kit
GeneBLAzer™PPAR gamma DA (Division Arrested) cells and PPAR gamma-UAS-bla HEK 293H cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-gamma (PPAR gamma) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293H cell line.
Marke: Thermo Scientific™ K1419
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Beschreibung
GeneBLAzer™PPAR gamma DA (Division Arrested) cells and PPAR gamma-UAS-bla HEK 293H cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-gamma (PPAR gamma) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293H cell line. GeneBLAzer™UAS-bla HEK 293H cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-PPAR gamma (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both PPAR gamma DA cells and PPAR gamma-UAS-bla HEK 293H cells are functionally validated for Z' and EC50 concentrations of Rosiglitazone. In addition, PPAR gamma-UAS-bla HEK 293H cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.
Spezifikation
| Zellbasiertes Beta-Lactamase-Reportergen | |
| Versand: Trockeneisenlagerung: -80 °C/Flüssigstickstoff Ausreichend „Division Arrested“-Zellen und Substrat, für den Assay einer 384-Well-Platte. Lieferumfang: PPAR-gamma 293H DA-Zellen (K1419A)LiveBLAzer™-FRET B/G Lade-Kit, 70 μg | |
| Fluoreszent | |
| 5468 | |
| PPAR gamma 293H DA-Zellen, LiveBLAzer™-FRET B/G Ladekit, 70 μg | |
| GeneBLAzer™ | |
| BLA (Beta-Lactamase) | |
| PPARG, PPAR-gamma, NR1C3 | |
| 293H | |
| Kernrezeptoren |
| Zellen mit arretierter Teilung | |
| GeneBLAzer™ PPAR Gamma DA-Assay Kit, 1 Platte, ∽14,000,000 Zellen/Fläschchen bei Zellen/ml Zellmenge, 293H-Zelllinie, Fluoreszenznachweisverfahren, Zellkern-Reporter Druggable Target, BLA (Beta-Lactamase)-Reporter, Lagerung in Flüssigstickstoff | |
| Zelluläre Bildgebung, Pharmzie und Biopharmzie, Ziel- und Verlaufserkennung und -validierung, zielgerichtete adme/tox-Assays, Zellanalyse, zellbasierte Reporterassays, zellbasierte und Lactamase-Assays, Arzneimittelforschung und -entwicklung, Kernrezeptorbiologie, zellkernbasierte Rezeptor-Reporter-Assays, zielgerichtete ADME/tox-Assays, zelluläre adme-Assays, Zellkern-Reporter-adme-Assays | |
| PPARγ | |
| Calcitriol | |
| 1 Platte | |
| Gehäuse 293H | |
| PPAR gamma DA Assay-Kit | |
| GeneBLAzer™ |
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.