missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Thermo Scientific™ GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1 Cells
GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1 cells contain the human Orexin Receptor B (HCRTR2) stably integrated into the GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cell line.
Marke: Thermo Scientific™ K1737
Dieser Artikel kann nicht zurückgegeben werden.
Rückgaberichtlinie anzeigen
Beschreibung
GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1 cells contain the human Orexin Receptor B (HCRTR2) stably integrated into the GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cell line. GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cells (Cat. No. K1537) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element and the Promiscuous G protein, Gα15.
The GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of Orexin B. In addition, GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.
The HCRTR2 receptor has been detected in the brain as well as adrenal gland, gastrointestinal tract, and pancreas. The signaling cascades used by orexin receptors appear to be varied and cell type dependant. In CHO cells, calcium release is seen after agonist treatment. In hypothalamic neurons, the frequency of action potentials is increased. Administering either orexin peptide into the bloodstream of rats caused an increase in feeding behavior. The orexin system is also thought to regulate neuroendocrine function and the sleep wake cycle. HCRTR2 has been directly implicated in narcolepsy in animal studies.
For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
Spezifikation
| NM_001526.2 | |
| Teilende Zellen | |
| GeneBLAzer™ HCRTR2-Gα±15-NFAT-bla CHO-K1-Zellen, 2 x 106 Zellen, ∽2,000,000 Zellen/Fläschchen bei 2,000,000 Zellen/ml Zellmenge, CHO-K1-Zelllinie, Fluoreszenznachweisverfahren, GPCRs Druggable Target, BLA (Beta-Lactamase)-Reporter, Lagerung in Flüssigstickstoff | |
| FRET | |
| GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1-Zellen (LiveBLAzer™ FRET B/G Ladekit, Lösung D und andere Materialien sind separat erhältlich; siehe Protokoll). Inhalt: GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1-Zellen, GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1-Assay-Protokoll, Analysenzertifikat | |
| GeneBLAzer™ | |
| BLA (Beta-Lactamase) | |
| HCRTR2 | |
| CHO-K1 | |
| GPCRs |
| Zellbasiertes Beta-Lactamase-Reportergen | |
| Inhalt: K1737 GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1-Zellen (LiveBLAzer™-FRET B⁄G Ladekit, Lösung D und andere Materialien sind separat erhältlich; siehe Protokoll). Enthält: • GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1-Zellen • GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1 Assay-Protokoll • Analysenzertifikat Lagerung: Die GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1-Zellen werden auf Trockeneis geliefert und sollten sofort nach Erhalt in flüssigem Stickstoff gelagert werden. |
|
| Fluoreszent | |
| HCRTR2 | |
| Auf Einzelzellen basierende Klone | |
| 2 x 106 Zellen | |
| CHO-K1 | |
| GeneBLAzer™ HCRTR2-Gα±15-NFAT-bla CHO-K1-Zellen | |
| GeneBLAzer™ |
For Research Use Only. Not for use in diagnostic procedures.