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Thermo Scientific™ GeneBLAzer™ Gα15-NFAT CHO-K1 DA Assay Kit
The GeneBLAzer™ Gα15-NFAT CHO-K1 DA (Division Arrested) cell line contains the promiscuous G protein, Gα15, stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line.
Marke: Thermo Scientific™ K1453
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Beschreibung
The GeneBLAzer™ Gα15-NFAT CHO-K1 DA (Division Arrested) cell line contains the promiscuous G protein, Gα15, stably integrated into the CellSensor™ NFAT-bla CHO-K1 (Cat. No. K1078) cell line. NFAT-bla CHO-K1 cells contain a beta-lactamase reporter gene under control of an NFAT response element stably integrated into CHO-K1 cells. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
This product can serve as a negative control in screening assays performed with specific G-protein coupled receptor (GPCR) DA cells that were made from this same background. These cells can also be used in other assays where a sensitive readout to intracellular changes in calcium is needed.
Especificaciones
| Zellen mit arretierter Teilung | |
| Fluoreszent | |
| Gα15 | |
| 1 Platte | |
| Zellen mit arretierter Teilung | |
| GeneBLAzer™ |
| Jedes Kit enthält ausreichende Mengen an DA (Division Arrested)-Zellen und Substrat zur Analyse von 1 x 384 Well-Platte. (Weitere Materialien sind separat erforderlich; siehe Protokoll). Lieferumfang: o GeneBLAzer™ G?15-NFAT-CHO-K1-DA-Zellen o LiveBLAzer™-FRET B/G Ladekit, 70 µg o Lösung D o GeneBLAzer™ G?15-NFAT-CHO-K1 Assay-Protokoll o LiveBLAzer™-FRET B/G Ladeprotokoll o Analysenzertifikat Die GeneBLAzer™ G?15-NFAT-CHO-K1-DA-Zellen werden auf Trockeneis geliefert und sollten sofort nach Erhalt in flüssigem Stickstoff gelagert werden. |
|
| GPCRs | |
| GeneBLAzer™ | |
| BLA (Beta-Lactamase) | |
| CHO-K1 | |
| GPCRs |
For Research Use Only. Not for use in diagnostic procedures.