Thermo Scientific™ EasyPep™ MS Probenvorbereitungs-Kit

Beschreibung

Die EasyPep Mini MS-Probenvorbereitungskits ermöglichen die effiziente und reproduzierbare Verarbeitung von kultivierten Säugetierzellen, Geweben und Plasma für die proteomische MS-Analyse. Das Kit enthält vorformulierte Puffer, ein Enzym-Gemisch in MS-Qualität, Peptid-Aufreinigungssäulen und ein optimiertes Protokoll zur Erstellung von MS-kompatiblen Peptidproben in weniger als drei Stunden. Die Kits sind für die Verarbeitung von Proteinproben von 10 bis 100 μg mit einer hohen Ausbeute an MS-fähigen Peptiden optimiert.

• Complete—includes pre-formulated reagents for lysis through digestion, peptide clean-up columns, and an optimized protocol for up to 20 samples
• Optimized—streamlined protocol and reagents minimize the number of steps and time time needed to process samples
• Flexible—reagents and protocol have been verified using cells, plasma, and tissue samples for 10 to 100 μg samples
• Time-saving—sample processing time reduced from more than one day to less than three hours
• Compatible—final preparation is ready for direct MS analysis and other downstream applications, including TMT labeling

The sample preparation of peptides for MS analysis is complex, with numerous steps and varied protocols, resulting in sample variability in both quality and quantity. This kit has been designed to improve reproducibility while saving hands-on and processing time. The number of steps and processing time have been reduced through the addition of Universal Nuclease to reduce viscosity from nucleic acids (replacing sonication), a rapid 'one pot' reduction/alkylation solution for cysteine modification (carbamidomethylation, + 57.02), and a trypsin/Lys-C protease mix for more complete digestion. In addition, the kit includes Peptide Clean-up columns and buffers to prepare detergent-free peptide samples for MS analysis. The optimized reagents and protocol produce high-quality peptides that are compatible with TMT labeling and other downstream applications, or are ready for MS analysis.